Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

Development of chemically defined media supporting high-cell-density growth of lactococci, enterococci, and streptococci.

Lactococcus lactis IL1403 was used as an experimental strain to develop a chemically defined medium for study of the physiology and metabolic pathways of lactococci. An experimental leave-one-out technique was employed to determine the necessity of each of the 57 chemical components used in medium development. A statistical experimental design approach including three fractional factorial desig...

Recent Advances in High Cell Density Cultivation for Production of Recombinant Protein

This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...

Highly efficient, chemically defined and fully scalable biphasic production of vaccine viruses

Vectorial vaccines are predicted to yield novel therapeutic and protective approaches. They consist of recombinant live carriers that express antigen from an unrelated pathogen in the recipient. Promising viral carriers are host-restricted pox viruses that trigger a strong immune response without ability to replicate in the human organism. A block in replication is an important safety feature t...

Chemically defined media supplemented with precursors for synnematin production.

result from other techniques, such as those requiring aeration of cultures. The exhaust air from such operationls must be sterilized and the most reliable means of doing this is incineration. The 1 cfm capacity of the incinerator described above is sufficient for mnost laboratory scale operations. If provision is made for cooling the exit air, the apparatus may be employed as a source of steril...

Efficient AAV Vector Production System: Towards Gene Therapy For Duchenne Muscular Dystrophy